Analysis of free amino acids in microbially colonized sandstone by precolumn phenyl isothiocyanate derivatization and high-performance liquid chromatography.
نویسندگان
چکیده
A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 57 3 شماره
صفحات -
تاریخ انتشار 1991